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Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI
Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1, Pages 87-96 doi: 10.15302/J-FASE-2016087
Keywords: safety of transgenic bicistron gene transfer body transgenic mice muscle development
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3, Pages 179-184 doi: 10.15302/J-FASE-2014020
Keywords: recombinant human butyrylcholinesterase (rhBCHE) human lactoferrin bacterial artificial chromosome (hLF BAC) transgenicmice milk
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3, Pages 382-389 doi: 10.15302/J-FASE-2018211
The mammary gland provides a novel method for producing recombinant proteins in milk of transgenicgenomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic
Keywords: BAC recombinant methods gene expression human lysozyme transgenic mice milk expression vector
Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO
Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2, Pages 158-173 doi: 10.15302/J-FASE-2014015
Keywords: bovine Ig μ heavy-chain transgenic mice B cell development allelic exclusion immune response Ig repertoire
Advances of Transgenic Breeding on Rapeseed (Brassica napus)
Guan Chunyun
Strategic Study of CAE 2002, Volume 4, Issue 8, Pages 34-39
Keywords: rapeseed transgenic breeding
Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US
Hu Wei and Zhu Zuoyan
Strategic Study of CAE 2016, Volume 18, Issue 3, Pages 105-109 doi: 10.15302/J-SSCAE-2016.03.018
Keywords: transgenic Atlantic salmon industrialization enlightenments
Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG
Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 66-72 doi: 10.15302/J-FASE-2018229
Keywords: piglet diarrhea RNAi PEDV TGEV transgenic resistance breeding
Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice
Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 3, Pages 298-306 doi: 10.1007/s11705-022-2212-9
Keywords: carbon dots two-photon imaging pH diabetic mice
HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang
Frontiers of Medicine 2008, Volume 2, Issue 2, Pages 186-190 doi: 10.1007/s11684-008-0035-9
Frontiers of Medicine 2021, Volume 15, Issue 5, Pages 750-766 doi: 10.1007/s11684-021-0839-4
Keywords: particulate matter 2.5 sirtuin 2 p65 airway inflammation bronchial hyperresponsiveness triptolide
Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu
Frontiers of Medicine 2016, Volume 10, Issue 4, Pages 490-498 doi: 10.1007/s11684-016-0470-y
Keywords: conjugate vaccine pneumococcal surface adhesin A Hemophilus influenzae b immunogenicity otitis media
Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX
Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen
Frontiers of Medicine 2016, Volume 10, Issue 2, Pages 212-218 doi: 10.1007/s11684-016-0438-y
Hemophilia B is a hemorrhagic disease caused by the deficiency of clotting factor IX (FIX). Gene therapy might be the ultimate strategy for the disease. However, two main problems that should be solved in gene therapy for hemophilia B are immunity and safety. Self-complementary adeno-associated virus serotype 8 (scAAV8), a non-human primate AAV featuring low immunogenicity and high transfection efficiency in liver cells, might be a potential vector for hemophilia B gene therapy. A strong liver-specific promoter-1 (LP1) was inserted and mutant human FIX Arg338Ala was introduced into plasmid scAAV8-LP1 to develop an optimized AAV8 vector that expresses human clotting factor FIX (hFIX). The efficiency of scAAV8-LP1-hFIX administered through normal systemic injection or hydrodynamic injection was compared. A high expression was achieved using hydrodynamic injection, and the peak hFIX expression levels in the 5×1011 and 1×1011 virus genome (vg) cohorts were 31.94% and 25.02% of normal level, respectively, at 60 days post-injection. From the perspective of long-term (200 days) expression, both injection methods presented promising results with the concentration value maintained above 4% of normal plasma. The results were further verified by enzyme-linked immunosorbent assay and activated partial thromboplastin time. Our study provides a potential gene therapy method for hemophilia B.
Keywords: hemophilia B AAV8 hFIX gene therapy
Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG
Frontiers of Medicine 2011, Volume 5, Issue 1, Pages 86-93 doi: 10.1007/s11684-011-0118-x
Keywords: adeno-associated virus angiogenesis gene transfer ischemia middle cerebral artery occlusion netrin-1
Frontiers of Medicine 2022, Volume 16, Issue 4, Pages 584-595 doi: 10.1007/s11684-021-0844-7
Keywords: hemophilia A adeno-associated virus (AAV) human/rat hybrid factor VIII gene therapy dual chain strategy
Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells
Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO
Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3, Pages 201-213 doi: 10.15302/J-FASE-2014017
Keywords: repertoire complementarity determining region 3 of the IgH chain (CDRH3) immunoglobulin heavy chain variable region
Title Author Date Type Operation
regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice
Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI
Journal Article
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
Journal Article
Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
Journal Article
conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenicmice
Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO
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Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US
Hu Wei and Zhu Zuoyan
Journal Article
Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine
Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG
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Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its
HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang
Journal Article
Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating
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Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate
Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu
Journal Article
Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX
Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen
Journal Article
Overexpression of netrin-1 improves neurological outcomes in mice following transient middle cerebral
Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG
Journal Article
Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed
Journal Article